A glass bead method for picking bacterial colonies
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چکیده
منابع مشابه
Glass bead transformation method for gram-positive bacteria
A simple, inexpensive and reproducible transformation method was developed for Gram-positive bacteria. It was based on agitation of bacterial protoplasts with glass beads in the presence of DNA and polyethylene glycol. By using this method, introduction of pGK12 into protoplasts of several strains of Gram-positive bacteria was achieved.
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Extraction of nucleic acids from Gram-positive bacteria is normally hampered by a thick and resistant cell wall. This paper presents procedures based on mechanical cell breakage to extract DNA from Staphylococcus aureus. The proposed system for DNA extraction involves bead-beating treatment and the GES method. These two steps allow a consistent extraction from the bacterium resistant to the GES...
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The emergent spatial patterns generated by growing bacterial colonies have been the focus of intense study in physics during the last twenty years. Both experimental and theoretical investigations have made possible a clear qualitative picture of the different structures that such colonies can exhibit, depending on the medium on which they are growing. However, there are relatively few quantita...
متن کاملCounting Bacterial Colonies
MALLIGO, JOHN E. (Fort Detrick, Frederick, Md.). Evaluation of an automatic electronic device for counting bacterial colonies. Appl. Microbiol. 13:931-934. 1965.-An automatic colony counter was tested extensively with colonies of two bacterial species, Serratia marcescens and Bacillus subtilis var. niger, grown on agar media. A stable relationship was established between machine counts and coun...
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MATERIALS Reagents AmpliTaq polymerase (Perkin Elmer) Bovine serum albumin (BSA) (nuclease-free; Boehringer Mannheim; stock 1.6 mg/ml) Dimethyl sulfoxide (DMSO) (Aldrich) DNA in ES PCR Lysis buffer (include positive and negative controls) dNTPs (Pharmacia; stock of the mix 10 mM) ES colonies in G418 selection (see Electroporating DNA into Embryonic Stem (ES) Cells and Selection) Light mineral o...
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ژورنال
عنوان ژورنال: Technical Tips Online
سال: 1997
ISSN: 1366-2120
DOI: 10.1016/s1366-2120(08)70064-3